Pregnancy diagnosis in dairy animals through inhibition of seed germination

Zakir Hussain, Sarzamin Khan, Arfan Yousaf, Naseer Ahmad, Rafiullah Rafiullah, Halima Sadia, Munibullah Munibullah


Pregnancy diagnosis is an important tool to increase the productivity of dairy animals and the income of the farmers. The present research was conducted to evaluate the validity of seed germination inhibition test for pregnancy diagnosis in cattle, buffalo, sheep and goats and to standardize this technique for pregnancy diagnosis in dairy animals. A total of 2400 urine samples were evaluated. Urine samples were collected early in the morning from all the experimental animals. Each sample was diluted as 1:4 and 1:8 with distilled water, making 25 ml of each dilution. Maize seed was used in present study to determine the pregnancy through Punyakoti test. Germination percentage of seeds was recorded. Pregnancy was confirmed per rectal in cattle and buffalo, and by ultrasonography in sheep and goat at day’s 40-60 post mating and insemination. Pregnancy diagnosis in buffaloes through inhibition of seed germination and shoot length of maize seeds differed significantly and 78.57 % of pregnancy was confirmed by rectal palpation. Pregnancy in goats and sheep was 60% and 66%, respectively confirmed by the ultrasonography method after 100% result of Punyakoti test at 1:4 dilutions. It was concluded that 1:4 dilutions of urine for pregnancy diagnosis through inhibition of seed germination was better than 1:8 dilutions in all animal species but it was mainly appropriate for the detection of pregnancy in cattle, in which detection rate was 92.3%.

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